DNA replication in vitro by recombinant DNA-polymerase-alpha-primase
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چکیده
منابع مشابه
Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of replication activity. The addition of purified pol alpha-primase complex isolated f...
متن کاملThe mouse DNA polymerase alpha-primase subunit p48 mediates species-specific replication of polyomavirus DNA in vitro.
Mouse cell extracts support vigorous replication of polyomavirus (Py) DNA in vitro, while human cell extracts do not. However, the addition of purified mouse DNA polymerase alpha-primase to human cell extracts renders them permissive for Py DNA replication, suggesting that mouse polymerase alpha-primase determines the species specificity of Py DNA replication. We set out to identify the subunit...
متن کاملMultiple phosphorylation sites of DNA polymerase alpha-primase cooperate to regulate the initiation of DNA replication in vitro.
DNA polymerase alpha-primase (pol-prim) is the only enzyme that can start DNA replication de novo. The 180-kDa (p180) and 68-kDa (p68) subunits of the human four-subunit enzyme are phosphorylated by Cyclin-dependent kinases (Cdks) in a cell cycle-dependent manner. Cyclin A-Cdk2 physically interacts with pol-prim and phosphorylates N-terminal amino acids of the p180 and the p68 subunits, leading...
متن کاملSpecies-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase.
Human cell extracts efficiently support replication of simian virus 40 (SV40) DNA in vitro, while mouse cell extracts do not. Since human DNA polymerase alpha-primase is the major species-specific factor, we set out to determine the subunit(s) of DNA polymerase alpha-primase required for this species specificity. Recombinant human, mouse, and hybrid human-mouse DNA polymerase alpha-primase comp...
متن کاملA Dna Polymerase-alpha/ Primase Cofactor with Homology to Replication Protein A-32 Regulates Dna Replication in Mammalian Cells
HOMOLOGY TO REPLICATION PROTEIN A-32 REGULATES DNA REPLICATION IN MAMMALIAN CELLS Darren E. Casteel, Shunhui Zhuang, Ying Zeng, Fred W. Perrino*, Gerry R. Boss, Mehran Goulian, and Renate B. Pilz From the Department of Medicine and Cancer Center of the University of California, San Diego, La Jolla, CA 92093, and *the Department of Biochemistry and Cancer Center of Wake Forest University, Winsto...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1994
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1994.tb18925.x